De novo assembly is used in case of completely unknown sequences. Without a reference sequence, you can count on millions of overlapping reads from NGS machines. The most developed de novo algorithms available today are included in the application to guarantee the success of your de novo sequencing projects.
BLAST is the most frequently used tool to learn more about the similarity between the query sequence and the sequences stored in the selected database. BLAST has different variations according to the different sequence comparison methods. We have extended BLAST to enable comparison to any custom databases.
A hybrid reference assembly application is used to assure the accurate alignment to the reference of the outputs from different sequencing platforms and to manage the joint result. Similarly to the standard reference assembly application, the alignment process involves the most suitable tools for the different datasets. The generated result files will be merged into a single BAM file.